FastQCFastQC Report
Mon 20 Mar 2023
SRR6503002_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503002_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124746070
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA43720193.5047348585811164No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC14545501.1660086766661266No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10276450.8237894788990147No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7311820.5861363007267483No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6451600.5171786173303896No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3664120.2937262873291319No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT2550190.20443048827109342No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2333390.187051183255713No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2110630.16919410767810159Illumina Single End PCR Primer 1 (100% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1616470.12958083569285989No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1462810.11726301277467097No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA1319430.1057692639134844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAA4873200.0142.663041
GATAGAC1936450.0141.880891
TATCAGC2459750.0141.86641
TGGAACA4361000.0141.086141
GGCTAAC4924000.0140.534322
ACCTTAC4375100.0140.200822
ATAGCGA952600.0140.139681
AACTCAC2464250.0139.9441
ATCAGCA2492000.0139.594422
TCACGCA2453100.0139.537062
TAAGGTC2129150.0139.439562
ATGAATC1701650.0139.373122
AGTTAGC1488500.0139.339612
GCTAACT2565250.0139.069273
TTCACGC2474500.0139.047841
ATAGACA2004200.0138.021152
CCTAATC1898350.0137.907871
ATCCGTC1801550.0137.633132
TGTTCTA4949850.0137.521772
TACGCAA1440750.0137.434842
Produced by FastQC (version 0.11.5)