FastQCFastQC Report
Mon 20 Mar 2023
SRR6503001_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503001_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115970619
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10943650.9436571171530955No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT5898810.5086469358243229No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3577520.30848503102324565No Hit
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT2642860.227890479742977No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1603160.13823846193319017No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1305400.1125629932181357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT2974850.0143.508761
CTGGCAT3419900.0143.183581
ATGCCTA2182700.0142.937231
AGAGTCA2304500.0142.412161
ACGTATC1901900.0141.709341
GAGCTGA3613450.0141.569351
CAAGACT1903050.0140.8891
GAACTTA3031200.0140.398972
AGCTGAA3663450.0139.93572
CTCAATG1810450.0139.693081
CAATGGA4341400.0139.506991
ACTTCGA1277500.0139.263812
AGGTACA3545900.0139.216932
AGCAGAT3596150.0139.215013
GAGTCAA2324600.0139.155262
GATCGCA3924350.0139.097032
GTCACTA1605300.0139.003192
ATCGCAT2190900.0138.984773
TGCCTAA2238150.0138.930372
ACACAGA5477400.0138.8141
Produced by FastQC (version 0.11.5)