FastQCFastQC Report
Mon 20 Mar 2023
SRR6503000_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503000_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111477826
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA39122233.5094180971918125No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC15656551.404454191634487No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9034570.8104365077948327No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8384670.7521379184412871No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA6802940.6102505084733174No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4179780.3749427262781389No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2591760.23249107853969092No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT2449560.2197351785457316No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2052280.18409759802814957Illumina Single End PCR Primer 1 (100% over 21bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1692250.15180148920378123No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1319200.11833743510570433No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA1138890.10216291803178867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG8510700.0143.857651
CGGATTG2897500.0141.5711
GAATGCG6915550.0141.386351
TATCAGC1873600.0141.028061
AGGATGA8643350.0141.00392
AACTCAC2479700.0140.776861
ATAGCGA1081050.0140.745711
ATGAATC2355250.0140.460422
GATAGAC1332350.0139.743411
CCTAATC1703950.0138.637311
GCGTGAC5750000.0138.363985
ATCAGCA1899650.0138.276252
TCACGCA1521200.0138.247832
GGATTGC2967400.0137.564542
GTCGTAG1086350.0137.544391
ATGCGTG6513450.0137.473953
TACGCAA1493650.0137.381582
AATGCGT6843900.0137.140822
TAGCGAC1106100.0137.130652
ATAGACA1360550.0137.128142
Produced by FastQC (version 0.11.5)