FastQCFastQC Report
Mon 20 Mar 2023
SRR6502999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104479530
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3870802737.04843140086867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA72267106.916866873348301No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAGGTGATAAAAAAAAAA14366821.3750846696955854No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA9134430.8742793923364701No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA7878770.7540969987135279No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7488610.7167537985670495No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5233450.5009067326393983No Hit
AAGCAGTGGTATCAACGCAGAGTACACGTCTGAAAAAAAAAAAAAAAAAA5064470.48473322956180986No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4893770.4683951009350827No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4384220.4196247820027521No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4338630.4152612478252917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4274400.40911363211530527No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT4125770.3948878789940958No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4116310.39398243847383313No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3827950.3663827737356782No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3789050.3626595563743443No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3239140.3100262797889692No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3172610.3036585252632741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3158950.3023510921230216No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3139710.3005095830733542No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2855590.27331573945633175No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2768210.2649523787099731No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2667610.2553236983359324Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2413320.23098495944612307No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2319870.22204062365135066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA2286750.2188706247051456No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2228720.2133164266722869No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2139820.20480758288250342No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2047920.1960116015070129No Hit
AAGCAGTGGGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2042610.19550336798031154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1982970.18979507277645677No Hit
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG1980370.1895462202021774No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1814710.17369048271943796No Hit
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1766930.16911733810441146No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1691640.16191114182845195No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1646350.15757632140956224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGGAAGAGCACACGTCTG1644740.15742222423856617Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAG1606780.15378897665408717No Hit
AAGCAGTGGTATCAACGCAGAGCACACGTCTGAACTCCAGTCACATACAC1594690.15263181218368804TruSeq Adapter, Index 27 (96% over 30bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA1407070.1346742275735735No Hit
AAGCTGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1372280.13134438870465823No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1336770.12794563681517326No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA1330920.12738571852304467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA1304370.12484455088953789No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA1233540.11806523249099608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1200740.11492586155393311No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1138440.10896297102408481No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA1126040.10777613566982928No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGA1074330.10282684081752665No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT249389550.042.8268781
AAGCTGT1045300.041.8866841
AGCAGTG256489300.041.6404382
AGTGGTA258145950.041.1326375
GTATCAA260808750.041.036949
GGTATCA261999450.040.8248528
GCAGTGG261975300.040.763043
CAGTGGT261637050.040.5821154
TGGTATC264071750.040.4999667
GTGGTAT264523750.040.408136
GGATCAA1959150.033.4115079
CAGTGGG2035050.031.9768094
AGTGGGA2092650.030.783995
AGCTGTG1434250.030.482922
TGTGGTA1404050.030.2755375
GTGGGAT2198000.029.7270226
GGGATCA2218700.029.4774598
GCTGTGG1819250.024.0060313
CTGTGGT1874600.022.7176824
GGGGAAA2166000.018.43949925-29
Produced by FastQC (version 0.11.5)