FastQCFastQC Report
Mon 20 Mar 2023
SRR6502998_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502998_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94553380
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA33252833.516831444840999No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8795420.9302068313158133No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA7627830.8067220865081712No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5109390.5403709523657431No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2545640.26922781607595625No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1931810.20430893110325618Illumina Single End PCR Primer 1 (100% over 21bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1548880.16381011445598243No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1291480.13658739645267046No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1193110.1261837493276285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACAT7856150.0142.292311
ATAGCGA1260500.0142.205691
AGTTAGC2902250.0141.552752
TAAGGTC3938050.0141.193222
CTAAGGT3939450.0140.965421
GAGTTAG2922600.0140.919251
CCACATA7924300.0140.912892
ATGAATC2303450.0140.7192
TAGCGAC1273400.0140.68682
GCGAGTA1563800.0140.521151
GTGCGAA1781100.0139.797292
GATAGAC596700.0139.394531
AGAGATC1572250.0138.845432
TCACGCA1321800.0138.333792
CGAGTAA1579750.0138.209032
TATCAGC1469000.0138.015381
ATCCGTC1578200.0137.938922
GTACGCA625850.0137.561161
GTTAGCT1252800.0137.122043
AGCGACT636600.0137.01643
Produced by FastQC (version 0.11.5)