FastQCFastQC Report
Mon 20 Mar 2023
SRR6502997_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502997_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100119347
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17878891.7857577516960832No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC14099071.4082263241289419No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT6831070.6822927041264063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGA3880100.0142.756031
AGGTACA8027400.0141.663882
ACGTATC2753550.0141.534521
ACGCTTA3106200.0141.316822
AAGGTAC8025300.0141.313861
AGAGTCA1298900.0140.846081
AGCTGAA3927150.0140.596882
CAGATCT3336050.0140.367661
AACGTGA1428950.0140.292481
AGTCACT1891500.0140.235461
ACGTGAT1431600.0140.215322
GTCACTA1896100.0139.812162
GGTACAT4210150.0139.80323
ACTTCGA1382950.0139.678742
AGATCGC2536150.0139.654271
AGATCTG3356750.0139.460192
GATCGCA2538150.0139.396482
GCTGATC2166800.0139.150912
AACATCG2348350.0138.607162
GAGTCAA1319400.0138.14312
Produced by FastQC (version 0.11.5)