FastQCFastQC Report
Mon 20 Mar 2023
SRR6502994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101177121
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1840899218.19481698831893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12921111.2770782438057315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA12026291.1886373007193989No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5000470.4942293228525449No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA4905310.4848240344771226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4184770.4136083294957562No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3771180.3727305108829891No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3093040.30570547663636327No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT2788170.27557317034154394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2614500.25840822254667634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2387690.23599109921303257No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC1829760.18084720951883973No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1794390.1773513598988451No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1657370.16380877253860585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1651780.16325627609032284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC1617140.15983257716929897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1582800.1564385292204549No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA1386640.13705074687784405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1341250.13256455478704518No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1274020.1259197719215592No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1235110.12207404082984334No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1163350.11498152828444289No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGAAAAAAAAAAAAAAAAA1132880.1119699778767178No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA1092860.10801453818793677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC1079700.1067138488749843No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1054670.10423996942945234No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1034220.1022187614925315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT128398750.072.050851
AGTGGTA134468000.068.875985
GTATCAA134610300.068.8348549
AGCAGTG134908400.068.676382
GGTATCA137085150.067.603418
CAGTGGT138789900.066.804664
GCAGTGG139573600.066.393083
GTGGTAT139731100.066.3296
TGGTATC140363100.066.067857
CGACCTA336050.022.9216345-49
ATGGGGG5344500.022.18205525-29
ATGGGGC4226900.021.95678325-29
ACACTCG437700.021.75043330-34
TACGCAC359050.021.53007550-54
AAACCGC708200.021.03069785-89
ATGGGAT6421250.020.98503925-29
GAAACCG734250.020.70625785-89
ATGGGGT2428600.020.68397125-29
ATGGGCT3274600.020.52735325-29
GATAAGT755000.020.271295-99
Produced by FastQC (version 0.11.5)