FastQCFastQC Report
Mon 20 Mar 2023
SRR6502992_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502992_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90360619
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA55296746.119561885692704No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8958600.9914274712969817No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8343050.9233059813368477No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA8320810.9208447321504073No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC7579460.8388012481410735No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4362500.48278775071250896No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA4275690.47318068947712716Illumina Single End PCR Primer 1 (100% over 21bp)
CCATGTACTCTGCGTTGATAGCACTGCTTCCCATGTACTCTGCGTTGATA3722360.41194494251970537No Hit
CCATGTACTCTGCGTTGATACCAGTGCTTCCCATGTACTCTGCGTTGATA2588110.2864201273344531No Hit
CCATGTACTCTGCGTTGATAGCAGTGCTTCCCATGTACTCTGCGTTGATA2539390.2810283980015675No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1787920.19786495707825993No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCTA1637800.1812515250697873No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1435190.15882914657767008No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1347150.1490859640968152No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1279380.14158601547428531No Hit
CCATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTT1246350.13793066202877605No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1164340.12885480565377713No Hit
GACAGTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT1045020.11564993816609423No Hit
CCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTGATAC1010710.11185293009114955No Hit
CCATGTACTCTGCGTTGATACCACTGTTCCCATGTACTCTGCGTTGATAC995700.11019180822566078No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC984970.1090043440273467No Hit
CATGTACTCTGCGTTGATACGACTGCTTCCCATGTACTCTGCGTTGATAC948490.10496718708843727No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCTTTGATA940920.10412943275654186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAATC1323850.0139.494252
ACATCGG6905100.0137.596681
AACTCAC1449000.0137.423921
TACGCAA1017900.0136.977082
TGGAACA2319350.0136.976331
AGATGTA2602700.0136.337451
CATCGGT5130200.0135.981782
GGTGACT3461500.0135.297886
CCTAATC508750.0135.028531
CGGTGAC5849700.0134.022845
CAACGGT1068250.0132.330522
TCGGTGA5071350.0132.322974
GGAACAA2401150.0131.936282
ATCGGTG6093700.0131.875083
TGACTGG2724100.0131.591958
ACCTTAC534450.0131.534742
TCACGCA993000.0130.038972
CATCGGG1564050.0129.868322
GATGAAT1438200.0129.816991
GTGACTG3061800.0129.092157
Produced by FastQC (version 0.11.5)