FastQCFastQC Report
Mon 20 Mar 2023
SRR6502991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76545831
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2177050328.441134828100566No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1025755813.400544309199544No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5841470.7631336577951581No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC5577710.7286758700157034Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5564260.7269187527665615No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC5223510.6824029384435059No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5198010.679071600907958No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT4705490.6147284494174476No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3809970.49773710079651495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG3750500.48996789910086674No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3479030.45450287161948766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAG3362150.43923358804478846No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA3301230.4312749573520209No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3225670.42140374699178584No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG3186280.416257810304522No Hit
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG3123950.40811497624214177No Hit
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC2999050.3917979543523409No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA2937860.3838040506739028No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2920590.3815478859978671No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2866040.3744214364855481No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2788860.36433858821129006No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2746400.35879158461288374No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2740400.3580077404868725No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2738810.35780002179347953No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2660210.3475316637427321No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2646770.3457758529004669No Hit
CAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2505490.3273189365466553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG2439760.318731924146202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGA2395150.31290404306930836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA2369850.30959883367129426No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG2279470.2977915283198114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA2163910.2826946904528347No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2121000.2770888985449776No Hit
AAGCAGTGGTATCAACGCAGAGCACACGTCTGAACTCCAGTCACATCACG2083090.2721363100754632TruSeq Adapter, Index 1 (100% over 32bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA2070740.27052289758275666No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2067010.270035607817753No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT1977040.2582818651482143No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1851640.2418995229145791No Hit
AGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT1842870.24075380408372601No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1836080.2398667538144566No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG1833540.23953492646777852No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA1811620.23667128259408404No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1802880.235529482983861No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1794110.23438376415300788No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAAAGCAGTGGTATCAACGCAGA1779160.2324306858723632No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC1660540.21693408750112073No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA1593380.20816025891730147No Hit
AACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA1582390.20672451775982417No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG1513420.19771422953132486No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT1513050.19766589247688748No Hit
CGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT1450860.18954134811078083No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1448130.18918469903344573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGCAAGCAGTGGTATCAACGCAGA1445990.18890512796183506No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1388620.1814102717102908No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC1388540.18139982045527733Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
CAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC1383200.1807021991831273No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGGAAGAGCACACGTCTG1347930.1760945021290578Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGG1304800.1704599692699136No Hit
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA1289380.1684454898660647No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG1249660.16325644175187018No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA1197830.15648533491000965No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC1124940.14696293518584963No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA1099330.14361722717465827No Hit
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT1074970.14043482002305258No Hit
AAGCAGTGGGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1070680.13987437147295456No Hit
TGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAT1060130.13849611221805141No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT1055570.13790039068228288No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCA1046350.13669588354197892No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG936780.12238158339413678No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC932280.12179370029962834No Hit
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTG906100.11837352709646591No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA832850.1088040967247452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAAAAAA797910.10423951109760635No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA782480.10222372528688076No Hit
CAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG774710.10120864714369616No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT200979900.034.7406771
AGCAGTG207765850.033.5970422
AGTGGTA209389850.033.17635
GTATCAA212521500.032.9364789
GCAGTGG212992250.032.772473
GGTATCA214361350.032.6196488
CAGTGGT213702100.032.4985284
GTGGTAT216259850.032.326916
TGGTATC216539700.032.2877167
AAGCTGT948100.026.5272731
GGATCAA1653400.023.2776039
AGTGGGA1671650.022.6207165
GGGATCA1852050.020.7655418
AGCTGTG1210650.020.7204552
CAGTGGG1961700.019.491714
TGTGGTA1284650.019.03615
GTGGGAT2025400.018.9359576
GATAAGT114800.017.23325295-99
ACCTAGC73700.016.55959535-39
CTACTTA127400.015.897329135-139
Produced by FastQC (version 0.11.5)