FastQCFastQC Report
Mon 20 Mar 2023
SRR6502985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133104643
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2832592421.280943595634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA54014374.058038005481146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA21716171.6315110810972988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA9854450.740353587815866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA8204380.6163857109026618No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6014360.451852006394698No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5678330.4266064557943332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG4164610.31288239885065466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3238430.2432995519172085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG3095910.232592186885622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2798010.21021129969147656No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2734490.20543911454689073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA2701310.2029463389943505No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2587770.19441620830612197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2581550.19394890680109483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2333420.17530718293575978No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC2305610.17321784935781692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA2259720.1697701860032035No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2197580.16510167868449188No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2119560.15924012507963378No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2069370.15546940762990513No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1935670.14542467913760151No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1722180.1293854189594273No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1668280.12533597344158762No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAG1647250.12375601352989617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTGTCAACGCAG1598220.12007244555698933No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1588730.11935947268195594No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1565110.11758492902460209No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA1511060.11352421417786306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA1479570.111158406397589No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC1472250.11060846314729983No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1472200.11060470670433338No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1467950.1102854090521846No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1463230.10993080083615116No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1420260.10670251375077879No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1338080.10052842409111153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT205406250.061.5858231
GTATCAA211286900.059.9198389
AGTGGTA211600450.059.7850655
AGCAGTG212937450.059.4254652
GGTATCA216974650.058.330028
CAGTGGT219336650.057.6966174
GCAGTGG220351600.057.4315573
GTGGTAT222933450.056.7598426
TGGTATC223057400.056.7408877
GGGGAAA5101650.021.13554425-29
ACCTTAC805450.020.84371855-59
ACACTCG388550.020.7943730-34
TATATCG270750.020.67808290-94
GGGCTAA939750.020.67361625-29
ATGGGGT3567200.020.58992625-29
GCTAAAC831150.020.29276330-34
TGGGACA1573550.020.16529825-29
TGGGACT1697600.019.93610825-29
GGGGGAA2378750.019.7209625-29
CATGGGG22050650.019.71096425-29
Produced by FastQC (version 0.11.5)