FastQCFastQC Report
Mon 20 Mar 2023
SRR6502984_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502984_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84630315
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19353272.286801130304194No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4299310.5080106342508592No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4185230.4945308309439708No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3902990.4611810791440396No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC3124190.3691573167369163No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1687070.19934582542910306No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA918570.10853912099937238Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC3053350.0142.895831
ATAGCGA1431550.0141.748491
TAGCGAC1439000.0141.119312
AGGCTAA3352200.0141.119031
ATAGACA3102850.0140.803652
GGCTAAC3368150.0140.744232
ATCCGTC2243000.0140.740622
ATGAATC2178650.0140.61262
GCTAACT1735650.0140.177673
AGTTAGC1948250.0139.922992
GTACGCA1132400.0139.899671
TACGCAA1135600.0138.644272
TAGACAT1572250.0138.517173
GATGTAC2157950.0138.446432
AGCGACT702200.0138.159943
GCGAGTA1234900.0138.08041
GAGTTAG1983750.0137.66731
AGAGATC1419200.0137.61152
CCTAATC1784900.0137.307371
GTGCGAA1247300.0137.21262
Produced by FastQC (version 0.11.5)