FastQCFastQC Report
Mon 20 Mar 2023
SRR6502981_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502981_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93550471
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC18578901.9859761048129836No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3565050.38108306263898983No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1818390.19437529074546295No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1422600.15206764699239195No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1309150.1399405033460494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAT3377300.0142.21042
AACGTGA3396650.0141.916951
ACGTATC1818450.0141.126891
GATCGCA3222500.0140.265842
CAAGCTA1839500.0140.145342
ATGCCTA1256150.0140.0921
AAGACTA2457700.0140.04662
ACGCTTA1710150.0140.046112
ACTAGTA5448500.0139.998762
AGGTACA3591050.0139.956512
ACTTCGA1153100.0139.937212
CAAGACT2462050.0139.92771
GTCACTA1416600.0139.62892
AGTCACT1430150.0139.211241
CGCATAC1578400.0138.949571
ACAAGCT1864150.0138.924931
AGACTAT1293000.0138.91413
AGATCGC3278850.0138.809451
ATCGCAT1825050.0138.799763
CCGAAGT1312100.0138.481611
Produced by FastQC (version 0.11.5)