FastQCFastQC Report
Mon 20 Mar 2023
SRR6502980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108653807
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1766176716.255083450504408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA26421722.431734398409068No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA18626791.7143246531619458No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA10814810.9953457038095316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA8331080.7667545417897783No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7324450.6741089154842039No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC6806540.6264428452102005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA6019660.5540220049537703No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3825990.35212664016457335No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG3116160.28679712989716044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2638480.2428336450281949No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA2210100.20340750692702372No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA1979360.1821712514868439No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1833440.1687414413376238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAG1589340.14627559253400113No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1553120.1429420692088589No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1495250.1376159787940058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA1458750.13425668554807288No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1407230.1295150201225807No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACACTCGCTTCTGGAACGT1344330.12372599148780862No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA1296230.11929908723768878No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1233020.1134815276191841No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1228390.11305540357182331No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1221930.11246085468500887No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1115590.10267380691041962No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1094800.10076039029170879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT109140050.060.9659841
AGCAGTG114609450.058.068222
AGTGGTA115205100.057.7535365
GTATCAA115776550.057.511219
GGTATCA118640800.056.105858
CAGTGGT119555900.055.6799164
GCAGTGG121636700.054.7172973
TGGTATC123062350.054.0984347
GTGGTAT123049250.054.0910846
ACACTCG1436350.026.22510130-34
GGGACAC1597550.024.88134625-29
GGTAAGG878850.024.472408110-114
CGCTTCT1585800.024.30022235-39
CTGGCGA936850.024.280682130-134
CTAGTCC1527650.024.27317870-74
TGGAACG1500350.024.18601840-44
CACTCGC1527200.023.9908630-34
ACGTCAA944800.023.82185695-99
CAGACGC1562100.023.798775-79
CTGCCGA1043650.023.78556880-84
Produced by FastQC (version 0.11.5)