FastQCFastQC Report
Mon 20 Mar 2023
SRR6502979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103560862
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19173781.8514504060423906No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT9462430.9137071493282858No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT4640100.4480553667079365No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2898820.27991462643483983No Hit
AACACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1871610.18072561041448262No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1798680.17368337470964657No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT1386510.13388359011534684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAT2575750.0141.835312
AACGTGA2583900.0141.373381
CGCATAC2871450.0140.950261
GTCACTA1683900.0140.554442
AGTCACT1693850.0140.359041
ATGCCTA1160800.0140.091831
AGAGTCA1772450.0140.009721
CGAACTT1863650.0139.779511
CTCAATG2425000.0139.549221
CAAGCTA1586600.0139.520682
AGTGGTC3000550.0139.452871
ACTAGTA3719000.0139.429112
CGACTGG4085250.0139.238081
ACGTATC1302500.0139.23381
GCATACA2886500.0139.08742
AACATCG2496100.0139.033222
GTGGTCA3003200.0138.933732
GAGTCAA1769800.0138.718932
GACTAGT3744750.0138.71191
ACAAGCT1608050.0138.397251
Produced by FastQC (version 0.11.5)