FastQCFastQC Report
Mon 20 Mar 2023
SRR6502978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6502978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102283117
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA31738903.103043877710532No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA12105151.183494437307772No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC9686970.9470741882064466No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8963480.876340129524993No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5664430.5537991181868265No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3657270.35756340902282047No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2366710.23138813808343367No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1747840.17088255141852982No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1380360.13495482348274546Illumina Single End PCR Primer 1 (100% over 21bp)
CCATGTACTCTGCGTTGATAGCACTGCTTCCCATGTACTCTGCGTTGATA1230740.12032679840994678No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1179300.11529762042742596No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT1084830.10606149204467438No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA1066810.10429971546526098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCAG6020650.0138.736621
CCTAATC2137300.0138.613111
GATAGAC1897450.0137.84931
TATCAGC2021650.0137.590791
ATGAATC1740550.0137.466812
ATAGCGA1088900.0137.3661
CAGTGAC4975000.0136.721655
GTGCGAA3540150.0136.279312
AGGCTAA1900150.0136.260481
TCAGTGA4169600.0136.2444
AGTTAGC1302700.0136.191222
ATCATTC1951450.0136.00131
GTCAGTG5343100.0135.883513
GGTCAGT5700950.0135.850142
ATAGACA1938300.0135.687742
GGCTAAC1906500.0135.55682
AACTCAC1724400.0135.327121
CTAATCC2195650.0135.248442
AGATGTA2920350.0135.174551
AGTGACT3209200.0135.15086
Produced by FastQC (version 0.11.5)