FastQCFastQC Report
Mon 20 Mar 2023
SRR7403978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88502134
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9224881.0423341882355064No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAATCCCAGCACTTTGGGA1330730.1503613460891237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACGCCTGTAATCCCAGCAC1205620.13622496379578825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCATGCCTGTAATCCCAGCAC991980.1120854328778106No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA979570.11068320680267438No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTGGGAGGCCGAGGCGGG978740.11058942375333006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT59253350.0135.199141
AGCAGTG60276600.0132.852942
AGTGGTA60314450.0132.805865
TGGTATC60378000.0132.642887
GGTATCA60703500.0132.29328
GTGGTAT60543750.0132.288366
CAGTGGT60656900.0132.031164
GTATCAA60855450.0132.018029
GCAGTGG62671450.0127.729923
CGGTATC80650.069.097037
AGCGGTA81350.068.590935
GCGGTAT82250.067.752856
AGGGGTA281400.038.1227955
CAGCGGT149200.037.7363974
AGCAGCG175950.032.1915742
AGGTATC237950.028.684387
CAACGCA60488100.027.11525510-14
AACGCAG60566750.027.06435410-14
TCAACGC60560050.027.05203410-14
ATCAACG60508200.027.0053910-14
Produced by FastQC (version 0.11.5)