FastQCFastQC Report
Mon 20 Mar 2023
SRR7403977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99069859
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG33034483.334463209440926No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9523320.9612731961191143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA9191570.9277867247191701No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8048090.8123651412484598No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA7039860.7105955404660463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6146750.6204460228413164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA4350370.4391214486335344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3860610.3896856257764533No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3023420.30518060997745033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1423900.14372686247590197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT1337840.13504006299231736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1214000.12253979285465624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT1096500.11067947517720803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT65047650.0112.319641
AGCAGTG66238350.0110.223092
GTATCAA66490850.0109.953819
AGTGGTA66563300.0109.773575
GGTATCA66668650.0109.5704048
CAGTGGT66780500.0109.387544
TGGTATC67056250.0108.910347
GTGGTAT67111050.0108.885136
GCAGTGG67465300.0108.230183
GTGACTG756950.039.0829161
TGACTGG758900.038.8621142
GACTGGA785900.037.391333
TGGAGTT1056150.027.9026096
AGTTCAG1075750.027.4410789
ACTGGAG1094800.027.002074
CTGGAGT1128400.026.2691755
CATGGGG19445650.026.13788425-29
ATGGGGG6749900.025.2258925-29
ATGGGGT3457250.024.64641825-29
ATGGGGC3987700.024.4930525-29
Produced by FastQC (version 0.11.5)