FastQCFastQC Report
Mon 20 Mar 2023
SRR7403975_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403975_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54909197
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA45417108.27131017778315No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10369461.888474165812332No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7048631.2836884138006972No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA6315501.1501716187909286No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5882511.0713159764474427No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC4499830.8195038801969732No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2421560.44101173069422234No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1403770.25565298286915394No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1363270.24827716930553548No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1178630.21465074420957206Illumina Single End PCR Primer 1 (100% over 21bp)
CCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGAT1118570.20371268587300595No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1072580.19533703980409695No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC989900.18027945300310982No Hit
AGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA871530.15872204432346734No Hit
CCATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTT852110.1551852961899989No Hit
CCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTGATAC848860.1545934099163752No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA692930.1261956171021769No Hit
CCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATA663380.12081400498353673No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT638890.11635391426321531No Hit
CCATGTACTCTGCGTTGATACCACTGCTTGCCCATGTACTCTGCGTTGAT630690.11486053966514936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAATC2763400.0142.894652
TGGAACA3028150.0141.857541
GATGAAT2790900.0141.60051
AGTTAGC634550.0140.941992
TCACGCA2283600.0140.787722
TGAATCG904800.0140.63933
ATGTTGC1975950.0140.554492
GGAACAA3059000.0140.080052
GACAGTG1194800.0140.06361
AGATGTA1613550.0140.041811
GATGTAC1619300.0139.925862
GGCTTCA1582600.0139.790572
ACAGTGC1203200.0139.703742
CACGCAT1103650.0139.688673
ATCCGTC1369950.0139.64262
GAATCGT369800.0139.61224
TTCACGC2311600.0139.59421
TAAGGTC715400.0139.566012
GAACAAT1354200.0139.562263
GAATCTA315850.0139.553914
Produced by FastQC (version 0.11.5)