FastQCFastQC Report
Mon 20 Mar 2023
SRR7403974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61160378
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7691791.2576426522412925No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3956960.64698095881618No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1494610.24437553345402802No Hit
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1388300.22699336488731314No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1066490.17437596608706377No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC972380.1589885530138483No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA649160.1061406128000059No Hit
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT639210.10451374254096338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA3088050.0142.790991
GATAGAC2203650.0142.400221
CGAGTAA3086550.0142.283982
GACTAGT1463650.0142.23971
CTCAATG4138900.0141.907671
ACTAGTA1470550.0141.57032
TCAATGA4154050.0141.160032
TCGTAGA1305150.0141.014942
ATAGACA2235300.0140.898482
CATACCA6402350.0140.894651
GTGCGAA1514200.0140.7512
CGTGAGA1425650.0140.696062
GCCACAT2834000.0140.455181
CCGAAGT1383800.0140.31591
ATACCAA6179800.0140.308762
TATCAGC1062050.0140.244171
GTCGTAG1315650.0140.240841
GAACTTA1309300.0139.886122
ATCAGCA1064900.0139.59922
TGCGAAT740950.0139.552873
Produced by FastQC (version 0.11.5)