FastQCFastQC Report
Mon 20 Mar 2023
SRR7403973_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403973_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102107638
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12560971.2301694805632464No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8179400.8010566261458326No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1702860.1667710695648449No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1429230.13997287842462874No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1421160.13918253598227392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA3815150.0141.706012
AGTCACT3835250.0141.383961
CAATGGA6090950.0141.237881
ATGCCTA2325000.0141.047931
AGAGTCA1938900.0140.999331
GAGTCAA1936000.0140.633242
CTCAATG4099600.0140.48121
GATCGCA2504500.0140.125722
CAAGCTA2254500.0139.996842
ATCCTGT4430850.0139.874661
GACGGAT1298450.0139.853413
GCTGATC3374750.0139.807222
AAGACTA1917800.0139.779192
ACGCTTA1719550.0139.615522
AGCAGAT1852150.0139.576423
ATCGCAT1400000.0139.430153
TGCCTAA2339150.0139.316652
TCAATGA4095900.0138.999472
TCCTGTA4456700.0138.762532
AACGTGA1140550.0138.65851
Produced by FastQC (version 0.11.5)