FastQCFastQC Report
Mon 20 Mar 2023
SRR7403970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96648813
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1154100911.941180281231183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11354561.1748266375501166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3575940.36999316277169386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3086660.31936864035774554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2975020.3078175414321953No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2967410.3070301546279725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2840370.2938856579645733No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2343680.24249444222351701No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1532710.15858549654407034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1342540.13890910382934551No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA1269440.13134563794384108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC1166210.12066469973097342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA1046960.10832621400120042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT94515750.075.067021
AGCAGTG97297800.072.9278952
GTATCAA97333700.072.9216469
AGTGGTA97445000.072.830525
GGTATCA98868650.071.775118
CAGTGGT99042750.071.652694
GTGGTAT100697200.070.483976
GCAGTGG100690400.070.474253
TGGTATC100779200.070.404697
ATGGGGG7542600.023.57133325-29
CATGGGG19242100.023.52680425-29
ATGGGGT3162700.023.1553925-29
ATGGGGC4576900.023.13458325-29
TCGTACG141500.022.21530750-54
TACGCAC316400.021.99102650-54
GAAACCG891450.021.44692485-89
CCTAACT935450.021.19825760-64
GGAATTC1632250.021.07035330-34
AAACCGC905850.020.95814985-89
ATTAGGG971200.020.85683645-49
Produced by FastQC (version 0.11.5)