FastQCFastQC Report
Mon 20 Mar 2023
SRR7403969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78231292
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1476253118.870365837751983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15536031.9859099348634046No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3678510.47020954223790656No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1760050.22498030583465245No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1618230.20685201006267417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA1481790.18941141864306676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC1413550.18068856641150705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1383190.17680776638586004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1375780.1758605750752525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1313610.16791362719664657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1151910.14724414879917874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1151190.1471521140159618No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1087370.13899425309248375No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT946520.12098994862567271No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT937730.11986635731389941No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG800910.10237719198092754No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG796150.1017687398029934No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA793270.10140060067012571No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG784210.10024249631464606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT107791700.066.990711
GTATCAA110875500.065.129599
AGTGGTA111078250.064.992175
AGCAGTG111200700.064.909992
GGTATCA113113300.063.8079878
CAGTGGT113296300.063.7171824
GCAGTGG114965750.062.7858123
TGGTATC115517650.062.4715587
GTGGTAT115635300.062.4373256
CATGGGG14016000.022.40625-29
ATGGGGG4914150.022.37771225-29
ATGGGGC2986300.022.17934825-29
ACACTCG384550.022.0142330-34
ATGGGGT2727750.021.58459925-29
ATGGGAT5444950.020.6336425-29
CGATAGG112050.020.3562135-39
TTAGGGC474450.019.37572345-49
TACGCAC178100.019.11347850-54
CACTCGC432900.019.08647730-34
ACGTCAA355300.019.012695-99
Produced by FastQC (version 0.11.5)