FastQCFastQC Report
Mon 20 Mar 2023
SRR7403968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91578731
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG61427836.707652456988075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9276491.0129524507169685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3159790.3450353554254863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630620.2872522878702043No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2094350.2286939311268683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2077410.22684415664156776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1840460.20097024493602123No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1490930.16280308579510674No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1387560.15151553039100313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1330260.14525861905642698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA1233560.13469939870645292No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA927520.10128115883151952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT81267900.0100.299851
AGCAGTG83624400.097.448992
AGTGGTA83655850.097.430615
GTATCAA83711950.097.373669
GGTATCA84195450.096.776688
CAGTGGT84557400.096.386384
GTGGTAT84844900.096.068246
TGGTATC84823750.096.0493247
GCAGTGG85784900.094.993923
CATGGGG21118750.025.33892325-29
ATGGGGG6964700.025.23741525-29
ATGGGGT5032850.024.84267825-29
ATGGGGC4562500.023.75906425-29
ATGGGAT7325350.022.97232825-29
CATGGGC8203100.022.61684625-29
ATGGGGA6189450.022.40918425-29
ATGGGCT2951250.022.27051725-29
ATGGGAG8026400.022.26330425-29
CATGGGT5300050.022.07231525-29
AAACCGC779000.021.38752485-89
Produced by FastQC (version 0.11.5)