FastQCFastQC Report
Mon 20 Mar 2023
SRR7403967_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403967_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205052838
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA24632681.2012845196514668No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21175961.032707481961308No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16384050.7990160077667396No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA6851790.33414753323238566No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2711690.13224347570356476No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2140730.10439894521235547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTC25010150.0143.923952
CTAAGGT25298800.0142.524661
GGCTAAC7355000.0142.493482
ATGCCTA5529450.0141.907461
AAGGTCG6372550.0141.817543
AGGCTAA7415350.0141.496831
AAGGTCA8154800.0141.049323
CAGATTC6515450.0140.90832
AAGGTCT11085900.0140.506133
TCACGCA5798300.0140.326372
ATAGCGA2062050.0140.268131
GCTAACT3665850.0139.973313
TGCCTAA5584800.0139.913392
TAGCGAC2070800.0139.39372
AGGTCTA3440050.0139.121464
GTCTGTC5590850.0138.108341
AGATTCG1798700.0137.987643
AGGTCAT3657000.0137.793014
TCTGTCA5611850.0137.759142
AGGTCGA2019850.0137.480094
Produced by FastQC (version 0.11.5)