FastQCFastQC Report
Mon 20 Mar 2023
SRR7403967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205052838
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3915523419.095192430353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA35532001.7328216642385608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA13640770.6652319535318989No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA9489500.4627831583584325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6939180.3384093615909866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA5528500.2696134349527998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3508430.17109882673264926No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAG3301920.16102776397564417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT2662090.12982458696816476No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2584150.12602361543515922No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA2546400.12418262652868037No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2435420.1187703629832229No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2260260.11022817445716113No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2212040.10787658544867348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT286169850.072.0885541
AGTGGTA296308650.069.633025
AGCAGTG296365250.069.59022
GTATCAA298258250.069.093949
CAGTGGT302061800.068.278224
GGTATCA302916750.068.009488
GCAGTGG304255950.067.769543
TGGTATC306155050.067.378487
GTGGTAT306445800.067.3289646
ATGGGCT9774450.022.40338125-29
ATGGGGG10067800.021.9618925-29
ATGGGGC6527050.021.7350425-29
ATGGGGT8246000.021.1610925-29
CATGGGG37130650.020.82519725-29
TGGGCAT4563050.020.41668925-29
CATGGGC36237600.020.17036225-29
TGGGCTT3758150.019.37551125-29
ATGGGAT12239950.019.23440725-29
GGGCTAA1238200.019.2148325-29
ATGGGCG5972400.018.8852925-29
Produced by FastQC (version 0.11.5)