FastQCFastQC Report
Mon 20 Mar 2023
SRR7403965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206099572
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4018853919.499574215515594No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA29017091.4079160727223636No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA9670520.46921591860462475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA6601420.3203024604049154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4258440.2066205164171811No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA3413710.1656340169401225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3104520.15063204498066593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT3061510.14854518960379015No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2595240.1259216588766133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA2480320.12034571328464476TruSeq Adapter, Index 2 (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2431820.11799248180874436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG2221720.10779838009561708No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2213610.10740488097665724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2102380.10200797505780361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT285909100.072.312951
AGTGGTA295650950.069.8828355
AGCAGTG296135300.069.755062
GTATCAA297421550.069.453929
CAGTGGT301286150.068.556444
GGTATCA301766800.068.394328
GCAGTGG303256550.068.0987243
TGGTATC305817300.067.497637
GTGGTAT306430400.067.425136
GTGACTG900600.023.1733991
ATGGGCT10779100.022.49042325-29
ATGGGGG9381550.021.641825-29
ATGGGGC6296700.021.20420525-29
CATGGGC38175050.020.93849425-29
TGGGCAT5123300.020.74065225-29
ATGGGGT8362450.020.6844625-29
ATGGGCG5982300.020.2784725-29
CATGGGG36723650.019.75773625-29
TGGGCTT4236200.019.56422825-29
TGGGCTA3144350.019.19291525-29
Produced by FastQC (version 0.11.5)