FastQCFastQC Report
Mon 20 Mar 2023
SRR7403964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162178149
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3847716323.725244884870403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA33621172.0731011056242847No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA9273260.5717946626706166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA7714940.4757077354483803No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3830740.23620568021158017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3693550.22774646416762345No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3254390.20066760041761236No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3224480.19882333223571322No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA2616600.16134109410756686TruSeq Adapter, Index 2 (95% over 22bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2503780.15438454658894893No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2458580.15159748801917822No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2283410.1407964028495602No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT1993540.1229228482562099No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1979010.12202691991508671No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1974600.1217549967227706No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG1894060.1167888529792013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1891300.11661866975679937No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1852520.11422747216087661No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1835810.11319712373829102No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1704710.10511342067419946No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1689590.10418111258625846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT254101050.061.5934141
AGTGGTA262457050.059.610855
AGCAGTG262737850.059.5344282
GTATCAA263202900.059.4203769
GGTATCA267131700.058.51428
CAGTGGT268314050.058.294314
TGGTATC270740300.057.7441027
GCAGTGG270907800.057.7275733
GTGGTAT271252750.057.6798326
ATGGGGC5028600.020.76527425-29
ATGGGGG6903250.020.73734325-29
ACCTAGC874100.020.07151235-39
GGGCTAA1156950.019.98152725-29
ATGGGCT6148200.019.9324425-29
ATGGGGT4768550.019.60553225-29
GGGGCTA750100.019.49175625-29
CATGGGG24816050.019.37806325-29
TAGCCCC930500.018.87349735-39
ATGGGAT9170400.018.05844125-29
TGGGATA2288050.018.03991725-29
Produced by FastQC (version 0.11.5)