FastQCFastQC Report
Mon 20 Mar 2023
SRR7403963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168185584
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5040307029.968721932790626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA38909492.3134854411778836No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA11327170.6734923250021239No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6405580.38086379626924505No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4092120.2433097952081315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3090320.18374464246590838No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3039530.1807247641391191No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2712340.1612706592022774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA2648260.15746058235288465TruSeq Adapter, Index 2 (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2492720.14821246510640293No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2380940.1415662355460858No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2157470.12827912765698157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2034180.12094853504209968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG1986860.11813497641985772No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1979240.11768190548364717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1952130.11606999563054107No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1928110.1146418113932999No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1912170.11369404883120066No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1813330.10781720744864791No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCAGTGGTATCAACGCAG1781930.10595022222594297No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1703980.10131546113964203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT287098600.049.7808341
AGTGGTA298401050.047.880155
GTATCAA299604700.047.6867569
AGCAGTG300614650.047.515152
GGTATCA305046650.046.795468
CAGTGGT307021850.046.5326164
GCAGTGG310349750.046.026433
TGGTATC311222650.045.867237
GTGGTAT311863750.045.820756
GATAAGT1420700.022.76694195-99
AAACCGC1400000.022.30102785-89
GAAACCG1432200.022.2430385-89
CGACCTA385200.021.69662545-49
CGATAGG239900.021.52461235-39
CTACTTA1573400.021.098818135-139
ATAGGCG707150.020.654612100-104
ATACAAC1597200.020.391048130-134
GGCGATA716300.020.298199105-109
TACAACT1674400.020.011627130-134
CAACTAC1628000.019.80537130-134
Produced by FastQC (version 0.11.5)