FastQCFastQC Report
Mon 20 Mar 2023
SRR7403962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97072801
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1117832011.515398633650223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA7394690.7617674491539602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5275010.5434076224914948No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5242780.5400874339661837No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2915720.3003642596034702No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2121350.21853186249359385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1878910.19355679249432597No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1055780.10876167053220191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT79042950.070.301961
AGTGGTA82395450.067.5138855
AGCAGTG82676900.067.2661362
GTATCAA82992900.067.047979
CAGTGGT83978650.066.234314
GGTATCA84080850.066.170668
GCAGTGG84842000.065.5465243
GTGGTAT85826900.064.820476
TGGTATC85877850.064.784027
GAAACCG1157750.023.08927785-89
GATAAGT1182100.022.87224295-99
AAACCGC1159650.022.79440985-89
CGCAGAT1251000.021.7795290-94
ATGGGGG4528850.021.75974325-29
CTACTTA1264200.021.71089135-139
CGACCTA289950.021.667845-49
ATGGGGC3924150.021.53762425-29
TACGCAC293450.021.50760750-54
CGATAGG125750.021.27611735-39
ATACAAC1272950.021.205023130-134
Produced by FastQC (version 0.11.5)