FastQCFastQC Report
Mon 20 Mar 2023
SRR7403960_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403960_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230478885
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8638930.37482522531293916No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4907300.2129175520785776No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2378520.10319904142195066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGATC7622350.0141.230642
GATCGCA4246450.0141.116742
AGATCGC4258700.0140.9011
GTACGCA2464850.0140.608581
CGCTGAT7642400.0140.455281
GTGCGAA4573350.0139.729432
GATGTAC3105200.0139.556492
GATAGAC2245000.0139.553281
CTGTAGC3959300.0138.897691
ATCGCAT2410700.0138.713353
ACTTCGA2634350.0138.490982
CTGATCG1799950.0138.393423
CTGGCAT5170850.0138.160981
GGTGCGA4639450.0137.53451
CTGATCA2624900.0137.22133
AGTGGTC4055450.0137.160661
GTGGTCA4063200.0137.070562
TGAGCCA4693250.0137.022192
TACGCAA2531700.0136.95982
CGAACTT4371450.0136.896061
Produced by FastQC (version 0.11.5)