FastQCFastQC Report
Mon 20 Mar 2023
SRR7403959_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403959_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133325258
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA98626937.397467777635952No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC16870041.265329634689325No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13046350.9785355150034662No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9217640.6913648725135038No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA7529800.5647692052469158No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC3744020.2808185077729233No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA3188410.239145233831087Illumina Single End PCR Primer 1 (100% over 21bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2304940.1728809705359805No Hit
CCATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTT1756460.13174247898323962No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1591950.11940348167186746No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA1367240.10254921089295774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1866750.0140.335531
GATAGAC1743000.0139.792541
TAGCGAC1873600.0139.414412
AGTTAGC1799200.0138.529972
ATCCGTC2195700.0136.906562
GGCTAAC1950500.0136.77242
AGAGATC2002850.0136.70742
GTCACTA1371400.0136.512482
TAAGGTC2147000.0136.251282
CTAACGA3462850.0136.250352
AGTCACT1385350.0135.809221
GATGTAC1761200.0135.781112
ACGCTTA1269600.0135.504972
TAACGAT1895350.0135.389773
ATAGACA1798600.0135.280362
ATGAATC1181050.0134.400452
AGCGACT925550.0134.152183
GCGAGTA1108900.0133.850561
GTCGTAG1096600.0133.53871
GTGCGAA1185400.0133.317862
Produced by FastQC (version 0.11.5)