FastQCFastQC Report
Mon 20 Mar 2023
SRR7403959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7403959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133325258
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2520683418.90627055827636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA16786071.2590315032429937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG10152270.7614663682105907No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6405740.4804595990356156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4429910.33226337353121793No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3428650.25716432515735316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2900730.21756792700149885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2425420.18191751783446763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG2389180.1791993532088271No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1994640.14960706095164655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAAGGCCAGTCCTGAGCA1724780.12936633507208364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCAGTGGTATCAACGCAG1679360.12595962874491493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGGAAGAGCACACGTCT1642390.12318671080313978Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA1601990.12015652728007473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1596140.11971775070557149No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1516030.11370913679386993No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1513700.11353437620949512No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1492690.11195853076841598No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1491130.1118415236818818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGGTGGTATCAACGCAG1469790.11024092674172811No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1353890.10154790024857856No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1341250.10059984282948096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT155950800.061.1704031
GTATCAA164425500.058.247689
AGTGGTA164741600.058.1454625
AGCAGTG167124250.057.030762
GGTATCA167832750.057.0132648
CAGTGGT170357500.055.9601974
TGGTATC172709050.055.4010627
GTGGTAT172931400.055.3911936
GCAGTGG173037300.055.0832753
AGGAGTG1283550.028.8410532
GAAACCG1957250.024.55025785-89
GATAAGT1978700.024.53798395-99
AAACCGC1941750.024.48591485-89
CTACTTA2088950.023.574757135-139
ATACAAC2113050.022.871523130-134
TACAACT2191600.022.671335130-134
CAACTAC2151950.022.395855130-134
CGCAGAT2224250.022.34646290-94
ACAACTA2192200.022.200752130-134
GCATTGA2179250.022.15961530-34
Produced by FastQC (version 0.11.5)