FastQCFastQC Report
Mon 20 Mar 2023
SRR6211809_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211809_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99142061
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4204420.4240803507201651No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4137740.41735464829604463No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGAT3400180.34296039094849967No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCGCGTTGATAC3072420.3099007594768481No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGAT1283660.12947683223974937No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1056090.10652290151603769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC2168550.0141.1991
ATGCCTA2105100.0141.033231
ACGTGAT1661100.0140.503782
CGAAGTA2827150.0140.41442
CCGAAGT2855100.0140.066831
CTCAATG2907700.0140.00961
AGAGTCA1852900.0139.804521
ACTTCGA1176250.0139.339982
AACGTGA1672150.0138.752211
AGTCACT1454150.0138.441
CAATGGA5369050.0138.33641
GAGTCAA1852900.0138.216372
GTCACTA1441950.0138.190782
ACGCTTA1277600.0138.101642
TGCCTAA2133150.0137.973852
ACTGTGT5770100.0137.376722
AACATCG1585100.0136.457442
CTGGCAT1488400.0136.39841
GATCGCA2109500.0136.311142
TGAGCCA2248500.0136.213262
Produced by FastQC (version 0.11.5)