FastQCFastQC Report
Mon 20 Mar 2023
SRR6211808_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211808_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76691164
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2423860.3160546630900008No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2056540.2681586629719168No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGATA1268150.16535803264115276No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGAT1266690.16516765868881583No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1019500.1329357838407564No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1002470.1307151890405523No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG931950.12151986635644231No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA908630.11847909884377293No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGGACCCCGCGTTGATA906200.11816224356693816No Hit
CCATGTACTCTGCGTTGATACCACTGCTCCCCACGGACCCCGCGTTGATA891430.11623633721350221No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA791930.1032622219686221Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA2226000.0140.88642
CCTAATC4160400.0140.831861
GCCACAT3234100.0140.61891
TTCACGC2749400.0140.593231
GTCTGTC1828800.0140.550431
TATCAGC1561050.0140.49321
TCACGCA2593100.0140.366332
AAGTGTC1695800.0140.117463
AACTCAC3226200.0139.944271
TCAAGTG3326650.0139.935131
CTAATCC3799850.0139.852052
ATCAGCA1505400.0139.203612
GGCTTCA2471300.0139.112272
GGTGCGA2394250.0138.993091
TGTTCTA3297550.0138.918122
TCTGTCA1770250.0138.874952
TGGAACA3769000.0138.839141
TGGCTTC2623950.0138.728851
GATGTAC1229050.0138.715682
AGTGTCA738550.0138.700414
Produced by FastQC (version 0.11.5)