FastQCFastQC Report
Mon 20 Mar 2023
SRR6211808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76691164
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1828251423.839140060515966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13578671.7705651201225736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA11656351.519907821453851No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10795671.407681072620048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC7225910.9422089355691615No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5070420.6611478735672861No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3909990.509835787601294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3524340.4595496816295551No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2560800.33391069667426093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA2522810.32895706211995945No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2268880.29584633765631724No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2259110.29457239689307624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA2219510.28940882941873197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCTAAAAAAAAACCAACC2106160.2746287694890118No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA2062440.26892798236834686No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1945690.2537045858372941No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1915510.24976932153487724No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1855330.24192226369129044No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1728750.22541710280991434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAAACCAA1695960.22114151246941566No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1688090.22011531863044875No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1622380.21154718684410634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAAAAAAAAAAAAAAAAA1592390.20763669723411682No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1472610.19201820955540588No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1459610.19032309902089894No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1447950.18880271526456424No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1431450.1866512288169208No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1391790.18147983775549423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC1354700.17664355700742787Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1268020.1653410815358077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA1239160.16157793614920227No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1236120.16118154107036373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGGAAGAGCACACGTCT1121950.14629455878385156Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1076970.14042947633445751No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA1066350.13904470142088338No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT1063500.13867308103447223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGAAAAAAAAAAAAAAAA1059550.1381580282182182No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAAAAAAAAAAAAAAAAA1037340.1352619970665721No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC991580.12929520798510766No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC982080.12805647336373718No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG975620.12721413382120525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAAAAAAAAAAAAAAA964860.12581110387110567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTTGTGGTGGTGGTGGT949770.12384347171989721No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACAACGTACAAAAAAAAAA933520.12172458355176353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAAAAAAAAAAAAAAAA915670.11939706639476745No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA907850.1183773922117025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCTAAAAAAAAAAAAAAA906660.1182222244012361No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA899850.11733424726739054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTAAAAAAAAAAAAAAA872460.11376277976430244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTG859190.11203246308792497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGAAAAAAAAAAAAAAAAA854740.11145221371265143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGGTTGTGGTGGTGGTGG849850.11081459136544074No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA848240.11060465844539796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGAAAAAAAAAAAAAAAAA840410.10958368033115262No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC832200.10851315283205247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAAAAAAAAAAAAAAAAA822830.10729136931602708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA799510.1042506018033577No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAGAGAAAAAAAAAAAAAAAAAA790430.1030666322915636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA780460.10176661290471481No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG779470.10163752371785621No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG770950.10052657435216396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT130253000.057.769531
AGCAGTG135201000.055.6307142
AGTGGTA135807850.055.3664365
GTATCAA135755350.055.2849859
GGTATCA137516050.054.608128
CAGTGGT138223450.054.2919464
GCAGTGG138968500.054.197983
TGGTATC139684550.053.6867457
GTGGTAT139782200.053.654036
TGGTAAC214200.034.4043737
GCATCAA368100.031.1055249
GTGGAAT414650.030.9111676
TGGAATC423500.030.2822027
CAGTGGA430400.030.0811614
TGCGAAT26500.028.8003043
GGTAACA261300.028.2304368
AGTAGTG172650.027.3990732
GCGGCAT53600.026.8533446
AGCGGTA187600.026.6999845
AGTGGCA343700.025.4198955
Produced by FastQC (version 0.11.5)