FastQCFastQC Report
Mon 20 Mar 2023
SRR6211807_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211807_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122899221
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30809172.5068645471723534No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9599650.7810993366670729No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5958410.4848208110285744No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC5300790.4313119283319135No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3117180.25363708367199495No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2540910.20674744553506974No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1683670.13699598632931936No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA1335900.1086988175458004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGC3436650.0140.787572
ATAGCGA2967500.0140.594421
TAGCGAC2949600.0140.154682
ATGAATC4086800.0139.489612
ATTGGCG4926850.0139.304441
TATCAGC2703200.0139.163211
TCACGCA4245050.0138.540452
TTCACGC4316750.0137.774951
TGGAACA7818300.0137.385761
TAAGGTC2387700.0137.376622
CTAACGA3295400.0137.325762
GAGTTAG3539350.0136.824571
ATCCGTC1580950.0136.394322
GATAGAC1704400.0136.306811
GCGAGTA2242350.0135.664281
GTCGTAG1544800.0135.62611
TAACGAT1827650.0135.555393
ATCAGCA2753150.0135.247312
ACCTTAC3037050.0135.182482
CGAGTAA2232850.0135.14262
Produced by FastQC (version 0.11.5)