FastQCFastQC Report
Mon 20 Mar 2023
SRR6211804_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211804_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172745647
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGA23698950.0143.384161
ACGTGAT23739500.0143.139252
TAGCGAC3330500.0141.959032
ATAGCGA3338250.0141.629461
CGTGATG10092800.0141.606193
CGTGATA4830000.0141.5523
CGCATAC4145450.0141.53441
GTACGCA2447250.0141.362351
AACATCG4463000.0140.964192
TACGCAA2456450.0140.83292
GCGAGTA2849750.0140.799441
ACCGAGA3541850.0140.606782
CATACCA6046750.0140.536471
GTGCGAA4699250.0140.465442
ATGCCTA4058750.0140.376681
GCATACA4194550.0139.877622
TCGTAGA3134750.0139.868122
ATACCAA6085800.0139.634722
GTCGTAG3140100.0139.629821
GAACTTA4932650.0139.469152
Produced by FastQC (version 0.11.5)