FastQCFastQC Report
Mon 20 Mar 2023
SRR6211804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172745647
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9766290.5653566483212165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT9529970.5516764193774446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA8918600.516285078951946No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5905370.34185347663203347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3752120.21720489431493462No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC3528420.2042552192357125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA3416160.1977566473787904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2553660.14782774815738192No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC2342670.13561383691480225No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1793350.10381448280430475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTCTTGCTTCAACA1788660.1035429853696979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT90227550.0137.229161
AGCAGTG92885450.0133.298862
GTGGTAT93226050.0132.768866
CAGTGGT93345350.0132.637744
AGTGGTA93387300.0132.562445
GGTATCA93632900.0132.298078
TGGTATC93829550.0131.982977
GTATCAA94122750.0131.739269
GCAGTGG94959100.0130.324923
CAACGCA93444550.027.25527610-14
ATCAACG93577800.027.09423810-14
CGCAGAG93426450.027.08423215-19
AGAGTAC94104000.027.01052515-19
AACGCAG94294750.027.00264510-14
GCAGAGT94070400.026.93293215-19
CAGAGTA94397300.026.92927215-19
TCAACGC94474950.026.8983210-14
ACATGGG92531500.026.85398320-24
TACATGG94535200.026.81477420-24
GTACATG94585100.026.76141220-24
Produced by FastQC (version 0.11.5)