FastQCFastQC Report
Mon 20 Mar 2023
SRR6211803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104227101
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2953550.28337639363105765No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1412720.1355424823722191No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1301990.12491856604550479No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1294110.12416252467772275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1119030.10736459032857491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT64373950.0140.999421
AGCAGTG65681100.0137.677842
AGTGGTA65725500.0137.360435
TGGTATC65694900.0137.072717
CAGTGGT65923400.0136.933334
GTGGTAT65969150.0136.817066
GGTATCA66279700.0136.183238
GTATCAA66758600.0135.657179
GCAGTGG67657050.0133.439623
CGGTATC90350.066.597337
GCGGTAT92900.065.393596
AGCGGTA96100.063.2915085
GCAGTTG647350.046.0258753
GTTGTAT644600.045.9756056
CAGTTGT694200.042.8682174
TTGTATC698950.042.348717
TGGTATA697000.038.140387
TGCGGCT246500.037.413145
AGTTGTA805600.036.913325
GGTATAA790450.033.6679658
Produced by FastQC (version 0.11.5)