FastQCFastQC Report
Mon 20 Mar 2023
SRR6211802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104888413
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4761920.4539986699960843No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2840800.270840211873546No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2329330.2220769609699405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1801760.1717787454749649No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1202720.11466662194612477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT53195750.0139.655461
AGCAGTG54220200.0136.58892
GTGGTAT54340650.0136.170756
TGGTATC54181900.0136.083277
CAGTGGT54414450.0135.9894
AGTGGTA54547550.0135.673135
GGTATCA54399250.0135.581928
GTATCAA54846750.0134.938199
GCAGTGG55715850.0132.84463
TGGTATA698050.042.653397
GGTATAA730050.040.793718
GCAGTTG711050.038.807723
GTTGTAT725550.038.0789266
TTGTATC784550.035.261697
CAGTTGT855150.032.5114364
TGTATCA888500.031.1770468
TGCGGCT320550.030.78094145
CAACGCA54373400.028.02987110-14
ATCAACG54441300.027.85332310-14
TCAACGC54730650.027.81906510-14
Produced by FastQC (version 0.11.5)