FastQCFastQC Report
Mon 20 Mar 2023
SRR6211801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54861871
Sequences flagged as poor quality0
Sequence length151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6999481.2758369104837857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4901490.8934237769615987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1148230.20929472128283777No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG942560.1718060253541116No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC744740.13574819568220703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA686330.12510145707571657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT23886150.0128.094331
GGTATCA24189150.0124.9143758
AGCAGTG24433500.0124.69952
GTGGTAT24268700.0124.5609056
AGTGGTA24295000.0124.53295
TGGTATC24220400.0124.527927
CAGTGGT24327550.0124.400854
GTATCAA24374700.0124.277879
GCAGTGG24671950.0122.8468863
GTCGTAG55200.048.3398481
CGGATTG121300.041.4256131
CGAGTAA62500.040.4866522
CCTTACG81950.032.3816223
TGACTGG326100.031.3053322
GTGATAT389950.031.1416666
GTGACTG340750.030.2168371
AGTGATA420000.029.3105775
GTTAGCG42800.028.9680183
GACTGGA378700.026.9188463
CATGGGG8955400.026.2215425-29
Produced by FastQC (version 0.11.5)