FastQCFastQC Report
Mon 20 Mar 2023
SRR6211800_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211800_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61837727
Sequences flagged as poor quality0
Sequence length151
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC3167650.0142.290731
ACCTTAC4475950.0142.168232
CCTAATC2759500.0141.181961
AGTTAGC2126250.0141.090772
GGATTGC4298250.0141.041212
CTAATCC2765050.0140.898592
CGGATTG4310500.0140.64041
CACCTTA4545750.0139.985231
GGCTAAC1352200.0139.571012
ATAGACA3238550.0139.175632
TAAGGTC1846600.0138.63922
ATGAATC1764050.0138.53512
CCTTACG1499800.0138.4613
CGACAAC1839350.0138.24762
TCACGCA1607350.0137.973342
TAGCGAC698900.0137.927412
AGCCATG1097000.0137.741731
CCGACAA1846900.0137.682461
AGGACAC1818350.0137.161042
GCCACAT1483400.0136.678311
Produced by FastQC (version 0.11.5)