FastQCFastQC Report
Mon 20 Mar 2023
SRR6211799_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211799_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33873696
Sequences flagged as poor quality0
Sequence length151
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAACA7861400.0143.555331
GGAACAA7900350.0142.847562
GAACAAT3119800.0141.368473
ATCCGTC1432050.0141.153872
ATCATTC2486700.0140.851381
CGACAAC1711050.0140.576282
TCACGCA1706450.0140.479422
CCGACAA1713550.0140.37121
GAACAAG2609100.0139.817443
AGTTAGC869300.0139.77062
GGCTAAC777850.0139.585542
GGCTTCA1689650.0139.340472
TAGCGAC521150.0138.800872
CCTAATC948550.0138.517721
ATAGCGA523450.0138.191011
CTAATCC951200.0138.131792
TCATTCC2536950.0138.06152
GATGTAC883900.0137.986832
CATTCCG843900.0137.895363
CAGATTC907200.0137.311682
Produced by FastQC (version 0.11.5)