FastQCFastQC Report
Mon 20 Mar 2023
SRR6211798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35466500
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10944363.0858302905558768No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1259740.355191518757137No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1199620.33824031127965826No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA972880.27430955972537463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC358450.10106720426317792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT21936500.0110.598851
AGTGGTA22190600.0108.364535
AGCAGTG22289850.0108.352312
TGGTATC22147150.0108.270317
GTGGTAT22213900.0108.190396
CAGTGGT22231700.0108.1636054
GGTATCA22210250.0108.113838
GTATCAA22396900.0107.660199
GCAGTGG22752500.0105.878353
CGCTGTT73850.041.524803145
TGCGGCT76500.037.14859145
GCAGTTG218200.035.524523
GTTGTAT241350.031.7803866
CAGTTGT242650.031.6760374
CCGCAAG172750.030.59336145
TTGTATC257800.029.5556557
TGGTATA244150.029.2779717
GGTATAA258500.027.9611748
ATGGGGT2234800.025.9026825-29
ACGTATC35350.025.641441
Produced by FastQC (version 0.11.5)