FastQCFastQC Report
Mon 20 Mar 2023
SRR6211797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37819029
Sequences flagged as poor quality0
Sequence length151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG23734406.275782490343684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4289241.1341486318963927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2679990.7086353274696714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA1576100.41674787578496525No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA928310.24546108785606316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT709000.187471761900603No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC652400.17250575100698645No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA642480.1698827328432996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG536480.14185451456196826No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT535440.1415795207222269No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT514760.1361113739858313No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT500410.13231698783170767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA478180.12643899450723603No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG474240.12539719092206203No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC462760.1223616819987631No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC451710.11943987245151112No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT413480.10933120466948001No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAGAGATGTTGTTGAAAA385930.10204651208786984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT29007450.077.148811
CGACTGG93200.076.477561
AGCAGTG29813100.074.77262
CGAAGTA61050.074.584112
AGTGGTA29972250.073.9187855
CAGTGGT30147850.073.48924
GTATCAA30200550.073.464899
GGTATCA30178500.073.310288
GTGGTAT30289300.073.105076
GCAGTGG30375050.072.986343
TGGTATC30290350.072.939157
CCGAAGT73000.063.3715321
ACGTATC56300.059.5017851
CGCATAC51800.059.4915921
AACCGAG86550.044.9887161
GACTAGT111550.044.396381
ACCGAGA94250.040.695522
CGCTCGA34100.039.7612042
ACGCTCG36650.036.9967881
CGTGATT62600.036.600333
Produced by FastQC (version 0.11.5)