FastQCFastQC Report
Mon 20 Mar 2023
SRR6211795_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211795_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34877464
Sequences flagged as poor quality0
Sequence length151
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGA3972200.0143.764192
GTCGTAG3985000.0143.302411
ATCAGCA2238100.0143.043912
CTCGGTA2431700.0143.011082
TATCAGC2242500.0142.763241
ACTTCGA1450400.0142.596452
GCGAGTA1609500.0142.112441
CCGTCTA2166100.0141.965122
GTGCGAA2640400.0141.923782
CGTAGAT1546300.0141.7513
GCCACAT2257100.0141.512181
CTCAATG2030700.0141.302861
CGTAGAG1303500.0141.019653
CGAGTAA1627100.0140.575242
CGTAGAA1182700.0140.264683
TCAATGA2053500.0139.733982
TGCGAAT1009350.0139.625413
CCACATA2288800.0139.552222
CACTTCG1483300.0139.433621
CGTCTAG770000.0139.115693
Produced by FastQC (version 0.11.5)