FastQCFastQC Report
Mon 20 Mar 2023
SRR6211795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34877464
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3728421.0690054758568455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1832340.5253650322741356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT488670.1401105309720913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA475150.13623410234184458No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA442080.12675233497481353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT434940.12470516778398796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT17945500.0128.887861
TGGTATC18082950.0126.787327
GTGGTAT18138200.0126.768626
AGTGGTA18153650.0126.7163545
GGTATCA18117450.0126.708358
AGCAGTG18212500.0126.635842
CAGTGGT18171600.0126.6090854
GTATCAA18247550.0126.156569
GCAGTGG18589000.0123.9109043
GTCGTAG37150.043.137261
TGGTATA244450.031.5255977
GGTATAA248250.031.1014358
GCAGTTG205100.030.0511973
GTTGTAT228450.027.0693976
CATGGGG7333550.026.7308425-29
ATGGGGT2248250.026.27273625-29
CAACGCA18147500.026.11960610-14
ATGGGGG2123500.026.09210425-29
AGAGTAC18099500.026.02517915-19
ACATGGG17463600.025.99400720-24
Produced by FastQC (version 0.11.5)