FastQCFastQC Report
Mon 20 Mar 2023
SRR6211791_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211791_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97797081
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9139080.9344941491658632No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3986050.40758373964147254No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3906710.3994710230666292No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3776430.38614956207128515No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1181020.1207622955535861No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1142460.11681943758628133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCT10087350.0140.469271
GGCTAAC3271850.0138.999572
AGGCTAA3263450.0138.901891
ATAGCGA1640750.0138.822191
GATAGAC1502250.0137.805181
TAGCGAC1664900.0136.947462
GCGAGTA1796600.0136.819271
TAAGGTC2355650.0136.50672
TCGTAGA2477850.0136.447752
CGAGTAA1836450.0136.18572
GTCGTAG2445350.0136.076521
AAGGAGC3440500.0135.945192
GCTAACT1673800.0135.042483
AGAGATC1948950.0134.766482
CAAGGAG3473250.0134.747211
ATGAATC1560800.0134.626722
TGGAACA2855450.0134.513981
GGCTTCA3249350.0133.723222
CTAAGGT2416700.0133.43121
TTGGCTC10651750.0133.296722
Produced by FastQC (version 0.11.5)