FastQCFastQC Report
Mon 20 Mar 2023
SRR6211790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85364142
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4973880.5826661972423972No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4845790.5676610678052618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT4792090.5613703702428123No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4139700.48494600929744014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT2316650.2713844414906671No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2049520.24009144261064558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1947440.22813325998169115No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT1909660.22370751409883555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC1818370.21301332824267125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1720730.20157527032837746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1347720.15787893703658382No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1303490.15269760457499826No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGTGGGGTTTTCTTCTA1278230.14973851667132085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA1135450.13301252415797724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC1101570.12904364457853978No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGCCCCTCATTTACATAAA1079370.1264430210052366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1058120.12395368537763785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT1022270.11975402974237122No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCAATAGATATAGTACCG1010280.11834945872237548No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACCCGTCTATGTA984510.11533062676363573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA942160.1103695272893389No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC920740.10786027697671932No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGCCAACTCCAGGGATTC905490.10607381258514845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT859200.10065116099919331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT41603850.0134.581251
GGTATCA41860700.0133.703258
GTGGTAT41910250.0133.528446
TGGTATC41992200.0133.273137
AGCAGTG42062600.0133.059282
GTATCAA42088950.0133.050969
CAGTGGT42096700.0132.955254
AGTGGTA42251450.0132.461855
GCAGTGG42430000.0131.858113
CGAAGTA77200.028.892192
CAACGCA41721650.027.19877410-14
TACATGG41693500.027.05486920-24
CGCAGAG41859050.027.03206415-19
ATCAACG41919450.027.02628510-14
GTACATG41834950.026.98688120-24
AGTACAT41864300.026.95690720-24
TCAACGC42093450.026.95291910-14
ACATGGG41561950.026.9503820-24
GCAGAGT41932200.026.94951615-19
CAGAGTA42069300.026.89931515-19
Produced by FastQC (version 0.11.5)