FastQCFastQC Report
Mon 20 Mar 2023
SRR6211785_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211785_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94681177
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA45103364.763709263986019No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9054150.9562777192767682No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8550670.9031013630090382No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA7784300.8221591922119853No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5347740.5648155387844408No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2315680.24457659625418474Illumina Single End PCR Primer 1 (100% over 21bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA2217360.23419227245136592No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1848930.19527957494655984No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1763430.18624926895448288No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1722560.18193267707265615No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA1094040.11554989435756592No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC963520.10176468338580118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAATC2866000.0141.060442
TATCAGC2827100.0139.568361
TGGAACA3613000.0138.790151
ATAGCGA1046700.0138.490841
GATAGAC1204500.0137.304081
CCTAATC2198450.0137.026181
GGCTAAC1110900.0136.9112
ATCAGCA2872400.0136.315862
GGAACAA3649700.0136.051852
TGAATCA960250.0135.628233
TACGCAA1266300.0135.521792
CTAATCC2217700.0135.520922
GATGTAC2267200.0135.001652
TAGCGAC1073900.0134.59332
ATAGACA1246950.0134.407852
TCACGCA1937750.0134.33912
GGCTTCA1866050.0133.924072
AACTCAC2311600.0133.705491
AGATGTA2266950.0133.50521
TAAGGTC812800.0133.057532
Produced by FastQC (version 0.11.5)